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300178 (WS), 300665 (SS) Protein Transport und Sortierung in Säugetierzellen
Seminar / Practical, 2 h
Winter and Summer Term
Contact: Huber Lukas (Lukas.A.Huber@i-med.ac.at)
 (held in english)
Location: 3., Dr. Bohrg. 9, SR Ebene 6
Lukas A. Huber (Biozentrum Med. Uni. Innsbruck), externer Lehrbeauftragter Biologie & Zellbiologie, 3 ECTS. Multimedia Übung / Vorlesung; Anmeldung online. Vorbesprechung nach Übereinkunft, Block-LV, beschränkt auf 30 Teilnehmer. Die VO wird aus didaktischen Gründen in Englisch gehalten, damit Kolleg(I)nen die wissenschaftliche Konversation in Englisch üben können.
Content: Subcellular fractionation has provided the means required to analyze the composition and properties of purified cellular elements. In
particular, subcellular fractionation has helped to define membrane boundaries and became necessary for the development of cell-free assays that reconstitute complicated cellular processes. Although cell fractionation techniques have improved over the last decades the purification of organelles to homogeneity is still a barely accessible
goal in cell biology. In this seminar, we will first briefly review the basic principles of subcellular fractionation, and the
establishment of different organelle fractions by density centrifugation, using tissue culture cells as a paradigm. Then we will discuss some of the intrinsic problems and will compare gradient
purification of cellular extracts with electromigration analysis. Finally, we will discuss alternative approaches, such as
immunoisolation and flow cytometry to purify organelles from tissue culture cells.
University: University of Vienna
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The ANA catalog of neuroscientific lectures is collected and maintained by  Michael Berger, Medical University Vienna. Technical implementation and database maintenance is done by Matthias Samwald.
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